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| Biotech-Billy |
| Good Luck Cloners |
| 2009.11.03 09:58:36 | |
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Oh my, is it already time for the cloning project? I see the menu is expanded this year. Should be good.
Best of luck, cloners.
Biotech Billy
Tags: Cloning Project | Biotech | MCC | BIO212AA Hits: 157 | Read more... |
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| Kikkert |
| proC and trp B gene sequences and primers |
| 2009.11.03 09:46:14 | |
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Scholars,
Visit the Documents section in the left hand menu and click on 212AA. In this folder are the sequences for and primers used to amplify the genes proC and trpB. Tags: Stan Kikkert | BIO212AA | Cloning Project Hits: 138 | Read more... |
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| Kikkert |
| And They're Off! |
| 2009.11.03 08:36:03 | |
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And They’re Off!
The students of BIO 212 AA have begun their cloning projects! Each student will PCR and subsequently clone the gene for T4 DNA Ligase as well as a biosynthetic gene from Agrobacterium radiobacter k84 into the plasmid vector pUC19. This semester we are looking at 2 A. radiobacter genes involved in proline (proC) and tryptophan (trpB) biosynthesis. Tags: Stan Kikkert | BIO212AA | Cloning Project Hits: 93 | Read more... |
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| Kikkert |
| E. coli lecture notes |
| 2009.08.31 08:04:41 | |
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Hi,
I just posted "E. coli Competent Cells" in the documents section. This lecture discusses E. coli cell structure, growth and the various strains available for making competent cells. (We will have this discussion on Monday while our cells are growing.) Tags: BIO212AA Hits: 126 | Read more... |
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| Kikkert |
| Welcome Fall 2009 |
| 2009.08.28 07:24:40 | |
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The doors to the lab have been thrown open and a new class of biotech recruits has walkwed hrough the door. Let's see if the website bbecomes a place where we can interact and talk molecular biology. Tags: BIO212AA Hits: 152 | Read more... |
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 londonundergroundAZ
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| Good Luck |
| 2009.04.03 06:28:16 | |
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To the students of BIO212AA, as you set sail on your quest to successful clone a gene, I wish you all the luck in the world; you are going to need it. For those of you wit facebook, check the MCCBiotechClub Page, where someone has been posting some links you may or may not find useful. They are not the ultimate answer that you seek, but they may asisst you on your way. Mcc Biotech's Profile  Tags: BIO212AA | PCR Pirates of Penzance | Cloning Project Hits: 124 | Read more... |
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| londonundergroundAZ
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| D-Day |
| 2008.12.17 12:45:16 | |
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Seasickness and fear As the beaches draw near. Overhead artillery booms Illuminating the Normandy morning with the first flashes of battle. Soldiers in landing craft, donning their gear Saying final prayers, as bullets pierce the air announcing the enemy.
Landing craft doors slap the angry surf, Men jump into too deep waters, some carried by the weight of their loads or murderous fire, to the bosom of Neptune.
Others bravely wade to shore, Amid the ordinance, obstacles, blood and bodies of their comrades. Then rush to meagre shelter by the sea wall, As the officer shouts that only the dead and the soon to be dead will remain on these beaches under fire.
The armada of a thousand ships at dawn grow fear and stubborn determination in the the German defenders, And hope in the hearts of French civilians. Their resistance emerges into the light, as they pray these liberators shall prevail against their oppressors.
D-Day, December 17 2008. The beginning of the end of the greatest conflict in history, The freedom we now enjoy born with from this day’s terrible labour, Leaving debts to the soldiers living and dead That can never be repaid. Except that we are thankful, and promise never to forget their great sacrifices. Tags: D-Day | BIO212AA | biotechnology Hits: 114 | Read more... |
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| londonundergroundAZ
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| Crushmode |
| 2008.12.03 10:21:09 | |
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Time is running out. BIO212AA is like a circus. Tags: has anyone seen the PCR Pirate? | londonunderground | UK | crushmode | cloning | island | Biotech | BIO212AA | LeuS
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| Kikkert |
| 2 Weeks to Go. Who's Gonna Get that Clone? |
| 2008.11.28 10:58:54 | |
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If this was a horse race we'd be turning for home. Everyone in the class now has PCR product. Hits: 118 | Read more... |
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| RaffaMolina |
| Perseverance |
| 2008.11.26 07:12:17 | |
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Well, for my first blog, I will go ahead and write about my final success with PCR, but not about the PCR itself, but about the way it made me feel.
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| Rbarlow83 |
| Coming down to the wire... |
| 2008.11.26 07:02:20 | |
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Is anyone interested in a heavy duty study group after the holiday? I would like to focus on BIO220 information, but BIO212AA could also be discussed at some point too... Hits: 117 | Read more... |
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| Rbarlow83 |
| Small Hurdle (Blue-White Selection) |
| 2008.11.23 06:06:08 | |
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This should prove useful on December 3rd... Tags: BIO212AA
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| Rbarlow83 |
| Out Sick |
| 2008.11.23 02:09:30 | |
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Sometimes science is interrupted by illness. Tags: BIO212AA Hits: 100 | Read more... |
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| londonundergroundAZ
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| Remember, Remember, The 5th of December |
| 2008.11.23 00:16:50 | |
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Not only are we going to be touring the facilities at TGen, I have designated this day a deadline for cloning. Tags: BIO212AA
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| Kikkert |
| 7 AM Mesa Community College Part II |
| 2008.11.21 21:59:20 | |
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It's 7 AM on a Friday. The campus is a little more desolate on Fridays, but half the 212AA class is waiting for me at the door to the laboratory. Tags: BIO212AA | Biotechnology Educator | Stan Kikkert Hits: 149 | Read more... |
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| Kikkert |
| ANY NEWS? |
| 2008.11.21 07:49:41 | |
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What's the latest from the folks working in the lab today? Tags: BIO212AA
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| londonundergroundAZ
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| Live From The Lab |
| 2008.11.19 05:28:05 | |
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Its about 3:10 In the afternoon, and im sitting in the lab still. After a busy morning of doing all these little itty bitty (not a waste of time) lab exercises for BIO220, at 11:30 I could finally sit down and get some real work done. Tags: PCR Pirate | Lab | BIO212AA | AJR | PCR
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| Rbarlow83 |
| LeuS PCR Plan |
| 2008.11.18 09:57:34 | |
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Wednesday PCR
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| Rbarlow83 |
| Many praises to Dr. K! |
| 2008.11.18 03:28:59 | |
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Dr. K was right! I guess he is a doctor... :)
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| Kikkert |
| I wish I saw this earlier |
| 2008.11.17 06:00:04 | |
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I don’t know why I didn’t think of this earlier. Sometimes troubleshooting is a round and round process. Suddenly you stare at something you’ve seen 1000 times and all of the sudden it looks different.
Josh did a great experiment yesterday. He ran a bunch of PCRs, some were done with the same buffers and reagents we have used in the past, some were done with a mastermix kit from Promega. (In earlier blogs we have called this ‘Dr. Obermiller’s Secret Sauce’.)
Tags: BIO212AA | Biotechnology Educator | Stan Kikkert | T4 | PCR
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