Are ghosts of the past rising?

First mass PCR attempt on T4 DNA Ligase gene nearly a complete failure.  A great chance to trouble shoot, however.

Tags: T4 | PCR

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Well, for my first blog, I will go ahead and write about my final success with PCR, but not about the PCR itself, but about the way it made me feel.

Tags: T4 | PCR | BIO212AA

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Its about 3:10 In the afternoon, and im sitting in the lab still. After a busy morning of doing all these little itty bitty (not a waste of time) lab exercises for BIO220, at 11:30 I could finally sit down and get some real work done.

Tags: PCR Pirate | Lab | BIO212AA | AJR | PCR

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Wednesday PCR

For Wednesday, I’m going to run a PCR with the LeuS template.

Tags: PCR | LeuS | BIO212AA

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Dr. K was right! I guess he is a doctor... :)

Tags: T4 | PCR | BIO212AA

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I don’t know why I didn’t think of this earlier. Sometimes troubleshooting is a round and round process. Suddenly you stare at something you’ve seen 1000 times and all of the sudden it looks different.

Josh did a great experiment yesterday. He ran a bunch of PCRs, some were done with the same buffers and reagents we have used in the past, some were done with a mastermix kit from Promega. (In earlier blogs we have called this ‘Dr. Obermiller’s Secret Sauce’.)

Tags: BIO212AA | Biotechnology Educator | Stan Kikkert | T4 | PCR

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In the lab today I talked with one of the students as he looked  at the results of his most recent PCR.  On one gel he had 4 or 5 rxns showing the desired product 1464 base pairs.

I went into lab today at 9:00am to run some gels. I wanted to look at my data from the PCRs I did on Friday.

Tags: T4 | PCR | BIO212AA

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Science... is science.

Nothing worthwhile is going to be done correctly on the first try. Walt Disney was fired by a newspaper editor because "...he lacked creativity...", and Thomas Edison attempted to create the light bulb 4,000 times before he finally succeeded! Abraham Lincoln lost 6 presidential elections before he was finally elected president, and when he did finally win the office of president, it was the only election he ever won!

Tags: R. Barlow | BIO212AA | PCR

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I surprised to hear that mr. Darland. I'm am somewhat grateful that i took a day off from PCR. I was really counting on the thermopol buffer solving the problems the class has been having.

Tags: BIO212AA | PCR | T4 | Biotech Billy

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